Luciferase Protein (AA 1-550) (His tag)

Produktnummer ABIN667203

Dieses Recombinant Luciferase-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Luciferase Protein (AA 1-550) (His tag) (ABIN667203)

Target: Luciferase

Protein-Typ: Recombinant

Spezies: Leuchtkäfer

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 90 by SDS - PAGE

Produktdetails

Proteineigenschaft: AA 1-550

Aufreinigungstag / Konjugat: Dieses Luciferase Protein ist gelabelt mit His tag.

Produktmerkmale: Luciferase, 1-550aa, Firefly, His tag, E.coli

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Lagerung: 4 °C

Antigendetails

Target: Luciferase

Hintergrund: Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562 nm. Luciferase from firefly is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. Recombinant firefly Luciferase, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Luciferin 4-monooxygenase, LUC. NCBI no.: BAF48390

Molekulargewicht: 62.9 kDa (571aa), confirmed by MALDI-TOF