NFNB (AA 1-217) protein (His tag)

Produktnummer ABIN667164

Dieses Recombinant -Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für NFNB (AA 1-217) protein (His tag) (ABIN667164)

Target: NFNB

Protein-Typ: Recombinant

Spezies: E. coli

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 95 % by SDS - PAGE

Produktdetails

Proteineigenschaft: AA 1-217

Aufreinigungstag / Konjugat: His tag

Produktmerkmale: NFNB, 1-217aa, E.coli, His tag, E.coli

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 50mM Nacl, 1mM DTT, 10% glycerol

Lagerung: 4 °C

Antigendetails

Target: NFNB

Hintergrund: NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E.coli NFNB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: dprA, nfsB, nfsI, ntr, dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive. NCBI no.: NP_415110

Molekulargewicht: 26.0 kDa (237aa) confirmed by MALDI-TOF