SAE1 Protein (AA 1-346) (His-T7)

Produktnummer ABIN667139

Dieses Recombinant SAE1-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für SAE1 Protein (AA 1-346) (His-T7) (ABIN667139)

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 90% by SDS-PAGE

Produktdetails

Proteineigenschaft: AA 1-346

Aufreinigungstag / Konjugat: Dieses SAE1 Protein ist gelabelt mit His-T7.

Sequenz: MVEKEEAG GGISEEEAAQ YDRQIRLWGL EAQKRLRASR VLLVGLKGLG AEIAKNLILA GVKGLTMLDH EQVTPEDPGA QFLIRTGSVG RNRAEASLER AQNLNPMVDV KVDTEDIEKK PESFFTQFDA VCLTCCSRDV IVKVDQICHK NSIKFFTGDV FGYHGYTFAN LGEHEFVEEK TKVAKVSQGV EDGPDTKRAK LDSSETTMVK KKVVFCPVKE ALEVDWSSEK AKAALKRTTS DYFLLQVLLK FRTDKGRDPS SDTYEEDSEL LLQIRNDVLD SLGISPDLLP EDFVRYCFSE MAPVCAVVGG ILAQEIVKAL SQRDPPHNNF FFFDGMKGNG IVECLGPK

Produktmerkmale: SAE1, 1-346aa, Human, His-T7 tag, E.coli

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Andere Bezeichnung: SUMO E1/SAE1

Hintergrund: SAE1, also known as AOS1, belongs to the ubiquitin-activating E1 family of proteins and plays an important role in the first step of the uBL1 conjugation pathway. Proteins conjugated to ub are marked for progressive degradation by the 26S Proteasome. SAE1, which is a dimeric enzyme, functions as a uBLI E1 ligase mediating the ATP-dependent activation of uBL1. This protein can bind with uBLE1A and uBLE1B to form a heterodimer which can bind uBL1. Recombinant human SAE1 protein, fused to His-T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 42.2 kDa (378aa) confirmed by MALDI-TOF

NCBI Accession: NP_005491