SlyD (AA 1-196) (Active) Protein

Produktnummer ABIN667072

Recombinant -Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für SlyD (AA 1-196) (Active) Protein (ABIN667072)

Target: SlyD

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: E. coli

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Reinheit: > 95 %

Produktdetails

Proteineigenschaft: AA 1-196

Sequenz: MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH

Produktmerkmale: SlyD,1-196aa, E.coli, Recombinant, E.coli

Aufreinigung: > 95% by SDS-PAGE

Biological Activity Comment: Specific activity is > 700nmol/min/mg, and is defined as the amount of enzyme cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 7.5)

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: SlyD

Hintergrund: SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. Recombinant E. Coli slyD was expressed in E. coli and purified by conventional chromatography.

Molekulargewicht: 21 kDa (196aa) confirmed by MALDI-TOF

NCBI Accession: NP_755987