SKP (AA 21-161) protein (His tag)
Kurzübersicht für SKP (AA 21-161) protein (His tag) (ABIN667019)
Target
Protein-Typ
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 21-161
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Aufreinigungstag / Konjugat
- His tag
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Sequenz
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ADKIAIVNM GSLFQQVAQK TGVSNTLENE FKGRASELQR METDLQAKMK KLQSMKAGSD RTKLEKDVMA QRQTFAQKAQ AFEQDRARRS NEERGKLVTR IQTAVKSVAN SQDIDLVVDA NAVAYNSSDV KDITADVLKQ VK -
Produktmerkmale
- Skp, 21-161aa, E.coli, His tag, E.coli
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Aufreinigung
- > 95% by SDS-PAGE
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- SKP
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Hintergrund
- The Seventeen Kilodalton Protein (Skp) is a trimeric periplasmic chaperone that assists outer membrane proteins in their folding and insertion into membranes. This protein required for the efficient release of ompA from the inner membrane, the maintenance of its solubility in the periplasm, and, in association with lipopolysaccharide (LPS), for the efficient folding and insertion of ompA into the outer membrane. Recombinant E. coli Skp, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molekulargewicht
- 17.9 kDa (162aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_414720
Target
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