His-Streptavidin (AA 25-183) protein (His tag)

Produktnummer ABIN666648

Produktdetails

Target: His-Streptavidin

Protein-Typ: Recombinant

Proteineigenschaft: AA 25-183

Spezies: Streptomyces avidinii

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: His tag

Applikation: SDS-PAGE (SDS)

Produktmerkmale: His-Streptavidin, 25-183aa, Streptomyces avidinii, His tagged, Recombinant, E.coli

Reinheit: > 95 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid in 20mM Tris pH 7.5

Lagerung: 4 °C

Referenzen

Lanz, Masi, Porciello, Cohnen, Cipria, Prakaash, Bálint, Raggiaschi, Galgano, Cole, Lepore, Dushek, Dustin, Sansom, Kalli, Acuto: "Allosteric activation of T cell antigen receptor signaling by quaternary structure relaxation." in: Cell reports, Vol. 36, Issue 2, pp. 109375, (2022) (PubMed).

Antigendetails

Target: His-Streptavidin

Hintergrund: Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin, the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant His tagged streptavidin was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Streptavidin, Streptomyces avidinii streptavidin, SA V1, SA V2, Streptavidin V1, Streptavidin V2. NCBI no.: CAA27265, P22629

Molekulargewicht: 17 kDa (167 aa), confirmed by MALDI-TOF.