GST Protein (AA 1-218)

Produktnummer ABIN6388215

Dieses Recombinant GST-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für GST Protein (AA 1-218) (ABIN6388215)

Target: GST (Glutathione S Transferase (GST))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Schistosoma japonicum

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Reinheit: > 90% by SDS-PAGE

Produktdetails

Proteineigenschaft: AA 1-218

Sequenz: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPK

Endotoxin-Niveau: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 30unit/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In Phosphate Buffer Saline containing 10 % glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: GST (Glutathione S Transferase (GST))

Andere Bezeichnung: Glutathione S-transferase/GST

Hintergrund: GST, also known as, Glutathione S-transferase, represents a major group of detoxification enzymes. GST acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. GST was originally separated from Schistosomajaponicum but currently isolated from recombinant E. coli source. Recombinant Schistosoma japonicum GST was expressed in E. coli and purified by conventional chromatography techniques.

Molekulargewicht: 25.4 kDa (218aa)

UniProt: P08515