PTH Protein (AA 32-115)
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- Target Alle PTH Proteine anzeigen
- PTH (Parathyroid Hormone (PTH))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 32-115
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- MSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ
- Reinheit
- > 95 % by SDS - PAGE
- Endotoxin-Niveau
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Measured by its ability to induce cAMP accumulation in MC3T3/E1 mouse preosteoblast cells. The ED50 for this effect is less than or equal to 50 ng/ml.
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Parathyroid Hormone (PTH) (Active) protein
PTH Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Parathyroid Hormone (PTH) (AA 32-115) protein (Fc Tag)PTH Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Parathyroid Hormone (PTH) (AA 32-115) (Active) protein (MBP tag,His tag)PTH Spezies: Ratte Wirt: Escherichia coli (E. coli) > 90 % CC, AcA Active
Parathyroid Hormone (PTH) (AA 32-115) protein (His-IF2DI Tag)PTH Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Parathyroid Hormone (PTH) (AA 32-115) protein (His tag)PTH Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Parathyroid Hormone (PTH) (AA 1-115) protein (GST tag)PTH Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Parathyroid Hormone (PTH) (AA 32-115) protein (GST tag)PTH Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Bioactivity Validated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Liquid. In phosphate-buffered Saline( pH 7.4) containing 2 mM EDTA
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PTH (Parathyroid Hormone (PTH))
- Andere Bezeichnung
- PTH (PTH Produkte)
- Synonyme
- PTH1 Protein, Pthp Protein, PTH-(1-84) Protein, Pth1 Protein, Pthr1 Protein, PTH Protein, parathyroid hormone Protein, parathyroid hormone S homeolog Protein, PTH Protein, Pth Protein, pth.S Protein
- Substanzklasse
- Hormone
- Hintergrund
- Human parathyroid hormone(hPTH) is an 84 amino acid residue peptide and one of the main regulators in maintenance of calcium homeostasis. It acts primarily on kidney and bone cells stimulating calcium back resorption or calcium mobilization, respectively. The whole mechanism is still under discussion, but low-dose hPTH triggers cyclic AMP-dependent protein kinase in some populations of bone cells bearing PTH receptors, which stimulates the proliferation of osteoblasts. Human parathyroid hormone(amino acids 32-115) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques
- Molekulargewicht
- 9.55 kDa (85 aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_000306
- UniProt
- P01270
- Pathways
- cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process
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