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Dihydrodiol Dehydrogenase (DDH) (AA 1-323) (Active) Protein Protein

DDH Spezies: Human Quelle: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Produktnummer ABIN6387732
$646.15
Zzgl. Versandkosten $45.00
100 μg ABIN6387732
100 μg ABIN6387732
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  • Target
    Dihydrodiol Dehydrogenase (DDH)
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 1-323
    Spezies
    Human
    Quelle
    Escherichia coli (E. coli)
    Applikation
    SDS-PAGE (SDS)
    Sequenz
    MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY
    Reinheit
    > 95 % by SDS - PAGE
    Endotoxin-Niveau
    < 1.0 EU per 1ug of protein (determined by LAL method)
    Biological Activity Comment
    Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Kommentare

    Bioactivity Validated

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    Liquid. In 20  mM Tris-HCl buffer (  pH 8.5) containing 0.1M NaCl, 20 % glycerol
    Lagerung
    4 °C,-20 °C,-80 °C
    Informationen zur Lagerung
    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • Target
    Dihydrodiol Dehydrogenase (DDH)
    Synonyme
    2-ALPHA-HSD, 20-ALPHA-HSD, C9, DD1, DD1/DD2, DDH, DDH1, H-37, HAKRC, HBAB, MBAB, aldo-keto reductase family 1 member C1, aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase), AKR1C1
    Hintergrund
    AKR1C1 also known as Aldo-keto reductase family 1 member C1, is member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant human AKR1C1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
    Molekulargewicht
    36.7kDa (323aa)
    NCBI Accession
    NP_001344
    UniProt
    Q04828
    Pathways
    Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process
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