NME2 Protein (AA 1-152)

Produktnummer ABIN6387709

Dieses Recombinant NME2-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für NME2 Protein (AA 1-152) (ABIN6387709)

Target: NME2 (Nucleoside diphosphate kinase B (NME2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Reinheit: > 90% by SDS-PAGE

Produktdetails

Proteineigenschaft: AA 1-152

Sequenz: MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE

Biological Activity Comment: Specific activity is > 1,800unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In 20 mM Tris-HCl buffer (  pH 8.0) containing 1  mM DTT, 10 % glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: NME2 (Nucleoside diphosphate kinase B (NME2))

Andere Bezeichnung: NM23-H2/NME2

Hintergrund: NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. Recombinant human NME2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 17.2 kDa (152aa) confirmed by MALDI-TOF

NCBI Accession: NP_001018149

UniProt: P22392