NAD Synthetase protein (His tag)

Produktnummer ABIN6253505

Recombinant -Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für NAD Synthetase protein (His tag) (ABIN6253505)

Target: NAD Synthetase

Protein-Typ: Recombinant

Spezies: Mycobacterium tuberculosis

Quelle: Escherichia coli (E. coli)

Reinheit: >98 % (SDS-PAGE)

Produktdetails

Aufreinigungstag / Konjugat: His tag

Verwendungszweck: NAD Synthetase (M. tuberculosis) (rec.) (His)

Spezifität: NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag.

Produktmerkmale: NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag.

Biological Activity Comment: ~0.1 U/mg protein. (Ammonium or glutamine as nitrogen source). One unit is defined as the amount of enzyme that synthesizes 1μmol NAD per min.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: 0.5 mg/mL (Lot specific)

Konzentration: Lot specific

Buffer: In 20 mM TRIS-HCl, pH 7.8.

Handhabung: After opening, prepare aliquots and store at -80 °C.Avoid freeze/thaw cycles.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung:

Short Term Storage: -20°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.

Haltbarkeit: 6 months

Antigendetails

Target: NAD Synthetase

Hintergrund:

NADE, Glutamine-dependent NAD(+) Synthetase, EC 6.3.5.1

NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It can use both glutamine or ammonia as a nitrogen source. Catalytic activity: ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate

Molekulargewicht: ~75.0kDa

UniProt: P0A5L6