LTA Protein (AA 35-205) (His tag)

Produktnummer ABIN6239734

Dieses not_set LTA-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für LTA Protein (AA 35-205) (His tag) (ABIN6239734)

Target: LTA (Lymphotoxin-alpha (LTA))

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Activity Assay (AcA), Cell Culture (CC)

Reinheit: > 97 %

Produktdetails

Proteineigenschaft: AA 35-205

Aufreinigungstag / Konjugat: Dieses LTA Protein ist gelabelt mit His tag.

Produktmerkmale: Tag location: N-terminal His Tag

Biological Activity Comment: Mechanism: TNF-β, a member of the tumor necrosis factor family, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells. The biological effects of TNF-β are very similar to TNF-α, due to the similarity of molecular structure and the receptors. As reported, TNF-α could inhibit the proliferation and induce apoptosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNF-β (10ng/mL) for 8h, 24h, 48h, 72h, then cells were observed by inverted microscope and IL-1β was detected in the cell supernatant by ELISA . Results 1: Cell apoptosis was observed after incubation with TNF-β (10ng/mL) for 72h.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 9.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: LTA (Lymphotoxin-alpha (LTA))

Andere Bezeichnung: Tumor Necrosis Factor Beta (TNFb)

Substanzklasse: Chemical

Hintergrund: Alternative Names: LT, LTA, TNF-B, TNFSF1, Tumor Necrosis Factor Ligand Superfamily Member 1, Lymphotoxin Alpha

Molekulargewicht: 23kDa

UniProt: P01374

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process