Thyroperoxidase Protein (His tag)

Produktnummer ABIN620907

Dieses Recombinant Thyroperoxidase-Protein wird in not_set produziert.

Kurzübersicht für Thyroperoxidase Protein (His tag) (ABIN620907)

Target: Thyroperoxidase (TPO) (Thyroid Peroxidase (TPO))

Protein-Typ: Recombinant

Spezies: Human

Wirt: Bitte anfragen

Applikation: Western Blotting (WB)

Reinheit: > 95 % as determined by SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses Thyroperoxidase Protein ist gelabelt mit His tag.

Produktmerkmale: Recombinant Human Thyroid Peroxidase

Anwendungsinformationen

Applikationshinweise: Western Blot: With monoclonal anti-TPO antibody and monoclonal anti-hexa-His-tag antibody. ELISA: 0.15-0.375 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: TPO is supplied in 16mM HEPES pH-7.6, 160mM NaCl, 0.08mM Kl and 20% glycerol. Immunological Function 1. Binds IgG-type human auto-antibodies. 2. Auto-antibodies to TPO recognize conformation-dependent epitopes. 3. Standard ELISA test (checker-board analysis of positive/negative sera panels, including international reference serum NIBSC 66/387).

Lagerung: 4 °C

Antigendetails

Target: Thyroperoxidase (TPO) (Thyroid Peroxidase (TPO))

Andere Bezeichnung: Thyroid Peroxidase

Hintergrund: Thyroid Peroxidase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain containing 834 AA and having a molecular mass of 92,872 Dalton (excluding glycosylation), 101 kDa total mass. The TPO is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. Introduction: Thyroid Peroxidase (TPO) represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. Its identity with the formerly so-called `microsomal antigen` has been shown several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx. 100 kDa molecular weight. The hemoprotein TPO plays a key role in the thyroid hormone biosynthesis by catalyzing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin (TG) to form precursors of the thyroid hormones T4 and T3. Synonyms: Thyroid peroxidase, EC 1.11.1.8, TPO, MSA, TPX.

Pathways: Thyroid Hormone Synthesis