Streptavidin Protein (AA 37-159) (His tag)

Produktnummer ABIN5853015

Dieses Recombinant Streptavidin-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für Streptavidin Protein (AA 37-159) (His tag) (ABIN5853015)

Target: Streptavidin

Protein-Typ: Recombinant

Spezies: Streptomyces

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 90% by SDS-PAGE

Produktdetails

Proteineigenschaft: AA 37-159

Aufreinigungstag / Konjugat: Dieses Streptavidin Protein ist gelabelt mit His tag.

Sequenz: AEAGI TGTWYNQLGS TFIVTAGADG ALTGTYESAV GNAESRYVLT GRYDSAPATD GSGTALGWTV AWKNNYRNAH SATTWSGQYV GGAEARINTQ WLLTSGTTEA NAWKSTLVGH DTFTKVKP

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 20 % glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: Streptavidin

Hintergrund: Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin, the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 Mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant Streptomyces streptavidin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 15.6 kDa (148aa) confirmed by MALDI-TOF