PDGFA (Platelet-derived growth factor subunit A) is a Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGFA has been described as a chemoattractant for monocytes and proven to be able to induce chemotactic migration of THP-1 cells. Therefore, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of PDGFA on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension,106cells/mL in RPMI 1640 with 0.5% FBS) and PDGFA (1.5ng/mL, 7.5ng/mL and 15ng/mL diluted separately in serum free RPMI 1640 ) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37oC with 5%CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (x40) and the number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter). Result shows PDGFA is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (x40) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (x400). Statistical results were shown in Figure 2. The optimum chemotaxis of PDGFA occurs at 1.5ng/mL.
PDGFA
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
Func, AbP, STD, PI
Active
Applikationshinweise
Isoelectric Point: 8.9
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Buffer
20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
Konservierungsmittel
Dithiothreitol (DTT), Other preservative, ProClin
Vorsichtsmaßnahmen
This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Target
PDGFA
(Platelet Derived Growth Factor A (PDGFA))
Andere Bezeichnung
Platelet Derived Growth Factor Subunit A (PDGFA) (PDGFA Produkte)