Interleukin 16 (IL16) (AA 1203-1332) (Active) protein (His tag)

Details zu Produkt Nr. ABIN5665840
Proteinname
Proteineigenschaft
AA 1203-1332
4
3
3
1
1
1
1
1
1
1
1
1
1
1
Spezies
Human
45
19
2
1
1
1
1
Quelle
Escherichia coli (E. coli)
60
2
1
1
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses IL16 Protein ist gelabelt mit His tag.
Applikation
Activity Assay (AcA), Cell Culture (CC)
Optionen
Produktmerkmale Tag location: N-terminal His Tag
Reinheit > 97 %
Biological Activity Comment Pro-IL16 (Interleukin16) is a 631 amino acid precursor molecule, which is then cleaved into different isoforms. Researches have shown that the recombinant human IL16, containing C-terminal 130 amino acids, has same bioactivity as the natural secreted human IL16. Besides, IL16 has been considered to stimulate the proliferation of Jurkat cells at low dose (10-9 M). Thus, a proliferation assay of recombinant human IL16 was conducted using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL16. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of Jurkat cells after incubation with IL16 for 72h observed by inverted microscope was shown in Figure 1. The CCK-8 data was shown in Figure 2. It was obvious that IL16 significantly promoted cell proliferation of Jurkat cells.
Proteinname
Hintergrund Alternative Names: LCF, PrIL-16, Lymphocyte Chemoattractant Factor, Pro-Interleukin 16
Molekulargewicht 17kDa
UniProt Q14005
Applikations-hinweise Isoelectric Point: 6
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
Konservierungs-mittel Dithiothreitol (DTT), Other preservative, ProClin
Vorsichtsmaßnahmen This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Bilder des Herstellers
Image no. 1 for Interleukin 16 (IL16) (AA 1203-1332) (Active) protein (His tag) (ABIN5665840) Pro-IL16 (Interleukin16) is a 631 amino acid precursor molecule, which is then cleave...
Image no. 2 for Interleukin 16 (IL16) (AA 1203-1332) (Active) protein (His tag) (ABIN5665840) Interleukin 16 (IL16) (AA 1203-1332) (Active) protein (His tag) (Image 2)
Image no. 3 for Interleukin 16 (IL16) (AA 1203-1332) (Active) protein (His tag) (ABIN5665840) Interleukin 16 (IL16) (AA 1203-1332) (Active) protein (His tag) (Image 3)