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YBX3/DBPA Protein

YBX3 Spezies: Borrelia burgdorferi Wirt: Escherichia coli (E. coli) Recombinant CP, ELISA, SDS, WB
Rockland
Produktnummer ABIN5624583
Hersteller Produkt- Nr.: 000-001-b98
  • Target Alle YBX3/DBPA (YBX3) Produkte
    YBX3/DBPA (YBX3) (Y Box Binding Protein 3 (YBX3))
    Protein-Typ
    Recombinant
    Spezies
    Borrelia burgdorferi
    Quelle
    Escherichia coli (E. coli)
    Applikation
    Control Peptide (CP), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Hersteller Produkt- Nr.
    000-001-b98
    Hersteller
    Rockland
    Verwendungszweck
    DbpA Control Protein
    Aufreinigung
    DbpA is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Decorin Binding Protein A and was estimated to be greater than 90% pure.
    Sterilität
    Sterile filtered
  • Applikationshinweise

    Application Note: DbpA is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect bands at 60.9 kDa for DbpA-MBP, (18.5 kDa for DbpA and 42.4 kDa for MBP) in size corresponding to DbpA by Western blotting in the appropriate cell lysate or extract. Decorin Binding Protein A has been tested in SDS-page and western blot.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Other: Lateral Flow Assay: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
    Haltbarkeit
    6 months
  • Target
    YBX3/DBPA (YBX3) (Y Box Binding Protein 3 (YBX3))
    Andere Bezeichnung
    DbpA (YBX3 Produkte)
    Hintergrund

    Synonyms: Decorin-binding Protein A, Borrelia burgdorferi dbpA, control protein, DbpA Control Protein

    Background: DbpA, or Decorin Binding Protein A is from the spirochete Borrelia burgdorferi, which is carried by Ixodes ticks. DbpA from other microbial organisms such as E. coli (ATP-dependent RNA helicase DbpA) are significantly different. The spirochete migrates from the tick midgut during tick feeding to tick salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix, enabling bacteria to colonize in mammalian tissues. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.

    Gen-ID
    1194347
    NCBI Accession
    WP_010890380
    UniProt
    O50917
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