YBX3/DBPA Protein

Produktnummer ABIN5624583

Hersteller Produkt- Nr.: 000-001-b98

Recombinant YBX3/DBPA-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für YBX3/DBPA Protein (ABIN5624583)

Target: YBX3/DBPA (YBX3) (Y Box Binding Protein 3 (YBX3))

Protein-Typ: Recombinant

Spezies: Borrelia burgdorferi

Quelle: Escherichia coli (E. coli)

Applikation: Control Peptide (CP), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Produktdetails

Hersteller Produkt- Nr.: 000-001-b98

Hersteller: Rockland

Verwendungszweck: DbpA Control Protein

Aufreinigung: DbpA is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Decorin Binding Protein A and was estimated to be greater than 90% pure.

Sterilität: Sterile filtered

Anwendungsinformationen

Applikationshinweise:

Application Note: DbpA is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect bands at 60.9 kDa for DbpA-MBP, (18.5 kDa for DbpA and 42.4 kDa for MBP) in size corresponding to DbpA by Western blotting in the appropriate cell lysate or extract. Decorin Binding Protein A has been tested in SDS-page and western blot.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

Other: Lateral Flow Assay: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Haltbarkeit: 6 months

Antigendetails

Target: YBX3/DBPA (YBX3) (Y Box Binding Protein 3 (YBX3))

Andere Bezeichnung: DbpA

Hintergrund:

Synonyms: Decorin-binding Protein A, Borrelia burgdorferi dbpA, control protein, DbpA Control Protein

Background: DbpA, or Decorin Binding Protein A is from the spirochete Borrelia burgdorferi, which is carried by Ixodes ticks. DbpA from other microbial organisms such as E. coli (ATP-dependent RNA helicase DbpA) are significantly different. The spirochete migrates from the tick midgut during tick feeding to tick salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix, enabling bacteria to colonize in mammalian tissues. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.

Gen-ID: 1194347

NCBI Accession: WP_010890380

UniProt: O50917