NADH Dehydrogenase Protein (His tag)

Produktnummer ABIN5505730

Dieses Recombinant NADH Dehydrogenase-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für NADH Dehydrogenase Protein (His tag) (ABIN5505730)

Target: NADH Dehydrogenase

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Antibody Production (AbP), Standard (STD)

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Produktdetails

Aufreinigungstag / Konjugat: Dieses NADH Dehydrogenase Protein ist gelabelt mit His tag.

Produktmerkmale:

• Recombinant human Purified recombinant protein of Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22 kDa (NDUFB10), nuclear gene encoding mitochondrial protein, full length, with N-terminal HIS tag, expressed in E. coli, 50 μg (full length, N-term HIS tag) protein expressed in E.coli.
• Produced with end-sequenced ORF clone

Aufreinigung: Purified

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the N-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: NADH Dehydrogenase

Hintergrund: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProtKB/Swiss-Prot Function]

Molekulargewicht: 20.6 kDa

NCBI Accession: NP_004539