IL23A Protein
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- Target Alle IL23A Proteine anzeigen
- IL23A (Interleukin 23, alpha subunit p19 (IL23A))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
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Spezies
- Maus
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Quelle
- Insektenzellen
- Applikation
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- Spezifität
- Optimal preservation of protein structure, post-translational modifications and functions.
- Produktmerkmale
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- Recombinant human Interleukin-23 alpha / IL23A protein expressed in Insect cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Reinheit
- Purity is > 95 % , as determined by Coomassie stained SDS-PAGE.
- Endotoxin-Niveau
- Less than 0.01 ng per μg protein as determined by the LAL method.
- Biological Activity Comment
- The ED50 is 0.5 - 0.8 ng/ml, corresponding to a specific activity of 1.25-2.0 x 10^6 units/mg, determined by mouse splenocytes IL-17A secretion induced by mIL-23 in a dose dependent manner. Under this assay, the bioactivity is equivalent
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Interleukin 23, alpha subunit p19 (IL23A) (AA 1-189) protein (GST tag)
IL23A Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 23, alpha subunit p19 (IL23A) (AA 20-157) protein (His-IF2DI Tag)IL23A Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 23, alpha subunit p19 (IL23A) (AA 22-196) protein (His-Avi Tag,Biotin)IL23A Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Interleukin 23, alpha subunit p19 (IL23A) (AA 22-196) protein (Fc Tag)IL23A Spezies: Maus Wirt: Mammalian Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
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- Applikationshinweise
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- > 50 μg/mL
- Buffer
- 1 x PBS
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- IL23A (Interleukin 23, alpha subunit p19 (IL23A))
- Andere Bezeichnung
- Interleukin-23 alpha / IL23A (IL23A Produkte)
- Molekulargewicht
- 55.4 kDa
- NCBI Accession
- NP_112542
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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