VEGF 165 (AA 27-191) (Active) protein (AVI tag,Biotin,His tag)

Details zu Produkt Nr. ABIN4949042, Anbieter: Anmelden zum Anzeigen
Proteinname
Proteineigenschaft
AA 27-191
4
3
1
1
Spezies
Human
33
5
4
Quelle
HEK-293 Cells
30
6
3
2
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
AVI tag,Biotin,His tag
Applikation
Functional Studies (Func)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Marke MABSol®,PrecisionAvi
Spezifität Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Produktmerkmale This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 22.4 kDa (monomer). As a result of glycosylation, the protein migrates as 30 kDa (monomer) under reducing (R) condition, and 48-60 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).
Reinheit >95 % as determined by SDS-PAGE.
Endotoxin-Niveau Less than 1.0 EU per μg by the LAL method.
Hintergrund VEGF165 is the most abundant splice variant of VEGF-A. VEGF165 is produced by a number of cells including endothelial cells, macrophages and T cells. VEGF165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability. VEGF gene expression is induced by hypoxia, inflammatory cytokines and oncogenes. VEGF165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix. VEGF165 binds to the receptor tyrosine kinases, VEGFR1 and VEGFR2. VEGF165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 that function to enhance VEGFR2 signaling. Binding of VEGF165 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin. VEGF plays a key role in tumor angiogenesis in many cancers.
Molekulargewicht 22.4 kDa (monomer)
Kommentare

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Lagerung -20 °C
Bilder des Herstellers
SDS-PAGE (SDS) image for VEGF 165 (AA 27-191) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949042) Biotinylated Human VEGF165, His Tag on SDS-PAGE under reducing (R) and no-reducing (N...
 image for VEGF 165 (AA 27-191) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949042) Measured by its binding ability in a functional ELISA. Immobilized Anti-VEGF MAb,Huma...
 image for VEGF 165 (AA 27-191) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949042) Measured by its binding ability in a functional ELISA. Immobilized Human VEGFR2/R3, F...
Produkt verwendet in: Keys, Wetter, Hang, Rutschmann, Russo, Mally, Steffen, Zuppiger, Müller, Schneider, Faridmoayer, Lin, Aebi: "A biosynthetic route for polysialylating proteins in Escherichia coli." in: Metabolic engineering, Vol. 44, pp. 293-301, 2017 (PubMed).

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