Protein L Protein (His tag)

Produktnummer ABIN2181671

Produktdetails

Target: Protein L

Protein-Typ: Recombinant

Spezies: Peptostreptococcus magnus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses Protein L Protein ist gelabelt mit His tag.

Marke: MABSol®

Produktmerkmale: Recombinant Protein L Protein is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 36-38 kDa polypeptide .Protein L Protein can bind to VL-Kappa.

Reinheit: >95 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Buffer: 100 mM NaH2PO4, pH 8.5

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.

Referenzen

Martinović, Andjelković, Klobučar, Černigoj, Vidič, Lučić, Pavelić, Josić: "Affinity chromatography on monolithic supports for simultaneous and high-throughput isolation of immunoglobulins from human serum." in: Electrophoresis, Vol. 38, Issue 22-23, pp. 2909-2913, (2017) (PubMed).

Antigendetails

Target: Protein L

Abstract: Protein L Produkte

Hintergrund: Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

Gen-ID: 17