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Protein L Protein (His tag)

Recombinant Protein L-Protein exprimiert in Escherichia coli (E. coli) und wurde in 1 Publikation erwähnt.
Produktnummer ABIN2181671

Kurzübersicht für Protein L Protein (His tag) (ABIN2181671)

Target

Alle Protein L Proteine anzeigen
Protein L

Protein-Typ

Recombinant

Spezies

  • 4
  • 1
Peptostreptococcus magnus

Quelle

  • 3
Escherichia coli (E. coli)

Reinheit

>95 % as determined by SDS-PAGE.
  • Aufreinigungstag / Konjugat

    Dieses Protein L Protein ist gelabelt mit His tag.

    Marke

    MABSol®

    Produktmerkmale

    Recombinant Protein L Protein is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 36-38 kDa polypeptide .Protein L Protein can bind to VL-Kappa.

    Endotoxin-Niveau

    Less than 1.0 EU per μg by the LAL method.
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  • Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

    Buffer

    100 mM NaH2PO4, pH 8.5

    Handhabung

    Avoid repeated freeze-thaw cycles.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
  • Martinović, Andjelković, Klobučar, Černigoj, Vidič, Lučić, Pavelić, Josić: "Affinity chromatography on monolithic supports for simultaneous and high-throughput isolation of immunoglobulins from human serum." in: Electrophoresis, Vol. 38, Issue 22-23, pp. 2909-2913, (2017) (PubMed).

  • Target

    Protein L

    Hintergrund

    Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

    Gen-ID

    17
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