IgE Protein

Produktnummer ABIN363702

Recombinant IgE-Protein exprimiert in Human.

Kurzübersicht für IgE Protein (ABIN363702)

Target: IgE

Protein-Typ: Recombinant

Spezies: Human

Quelle: Human

Applikation: Western Blotting (WB), ELISA, Isotype Control (IsoC), Flow Cytometry (FACS), Functional Studies (Func)

Reinheit: > 95 %, tested by analytical Gel Permeation Chromatography

Produktdetails

Spezifität: Binds with Fc effector domain to human and non human primate IgE receptors, no cross-reactivity with rodent or canine IgE receptors, specific antigen unknown

Produktmerkmale: IGHE, chromosome 14q32.33
Source of protein: Human B cell hybridoma from healthy donors (CD40 cell culture system)

Aufreinigung: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B

Sterilität: Sterile filtered

Anwendungsinformationen

Applikationshinweise: For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions < 1:100 Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS, pH=7.2, sterile filtered, contains no NaN3

Konservierungsmittel: Azide free

Lagerung: 4 °C

Antigendetails

Target: IgE

Substanzklasse: Antibody

Hintergrund: Recombinant human IgE to be used as reference for IgE concentration measurements in biological samples (ELISA, FACS). IgE (SUS-11) recognizes the human high affinity IgE receptor (FceRIa), the human low affinity IgE receptor (CD23) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with either anti-human IgE mAbs mAb LE27 or a specific recombinant cross-linking agent.