TRPV1 Protein (AA 1-839) (Strep Tag)

Produktnummer ABIN3135889

Dieses Recombinant TRPV1-Protein wird in Cell-free protein synthesis (CFPS) produziert.

Kurzübersicht für TRPV1 Protein (AA 1-839) (Strep Tag) (ABIN3135889)

Target: TRPV1 (Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Cell-free protein synthesis (CFPS)

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Reinheit: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-839

Aufreinigungstag / Konjugat: Dieses TRPV1 Protein ist gelabelt mit Strep Tag.

Sequenz: MEKWASLDSD ESEPPAQENS CPDPPDRDPN SKPPPAKPHI FATRSRTRLF GKGDSEEASP MDCPYEEGGL ASCPIITVSS VVTLQRSVDG PTCLRQTSQD SVSTGVETPP RLYDRRSIFD AVAQSNCQEL ESLLSFLQKS KKRLTDSEFK DPETGKTCLL KAMLNLHNGQ NDTIALLLDI ARKTDSLKQF VNASYTDSYY KGQTALHIAI ERRNMALVTL LVENGADVQA AANGDFFKKT KGRPGFYFGE LPLSLAACTN QLAIVKFLLQ NSWQPADISA RDSVGNTVLH ALVEVADNTA DNTKFVTNMY NEILILGAKL HPTLKLEELT NKKGLTPLAL AASSGKIGVL AYILQREIHE PECRHLSRKF TEWAYGPVHS SLYDLSCIDT CEKNSVLEVI AYSSSETPNR HDMLLVEPLN RLLQDKWDRF VKRIFYFNFF VYCLYMIIFT TAAYYRPVEG LPPYKLNNTV GDYFRVTGEI LSVSGGVYFF FRGIQYFLQR RPSLKSLFVD SYSEILFFVQ SLFMLVSVVL YFSHRKEYVA SMVFSLAMGW TNMLYYTRGF QQMGIYAVMI EKMILRDLCR FMFVYLVFLF GFSTAVVTLI EDGKNNSLPV ESPPHKCRGS ACRPGNSYNS LYSTCLELFK FTIGMGDLEF TENYDFKAVF IILLLAYVIL TYILLLNMLI ALMGETVNKI AQESKNIWKL QRAITILDTE KSFLKCMRKA FRSGKLLQVG FTPDGKDDFR WCFRVDEVNW TTWNTNVGII NEDPGNCEGV KRTLSFSLRS GRVSGRNWKN FALVPLLRDA STRDRHSTQP EEVQLKHYTG SLKPEDAEVF KDSMAPGEK
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: TRPV1 (Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1))

Andere Bezeichnung: Trpv1

Hintergrund: Transient receptor potential cation channel subfamily V member 1 (TrpV1) (Osm-9-like TRP channel 1) (OTRPC1) (Vanilloid receptor 1),FUNCTION: Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli (PubMed:15194687, PubMed:15489017). Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia (PubMed:10764638). Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions ( pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis (By similarity). {ECO:0000250|UniProtKB:O35433, ECO:0000269|PubMed:10764638, ECO:0000269|PubMed:15194687, ECO:0000269|PubMed:15489017}.

Molekulargewicht: 95.0 kDa

UniProt: Q704Y3

Pathways: Dicarboxylic Acid Transport