MCM9 Protein (AA 1-1134) (Strep Tag)

Produktnummer ABIN3134936

Dieses Recombinant MCM9-Protein wird in Cell-free protein synthesis (CFPS) produziert.

Kurzübersicht für MCM9 Protein (AA 1-1134) (Strep Tag) (ABIN3134936)

Target: MCM9 (Minichromosome Maintenance Deficient 9 (MCM9))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Cell-free protein synthesis (CFPS)

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Reinheit: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-1134

Aufreinigungstag / Konjugat: Dieses MCM9 Protein ist gelabelt mit Strep Tag.

Sequenz: MNSEQVTLVG QVFESYVSEY HKNDILLILK ERDEDAHYPV VVNAMSLFET NMEIGDYFTV FPNEVLTVFD SALRRSALAI LQSLPETEGL SMKQNLHARI SGLPVCPELV REHIPKTKDV GHFLSVTGTV IRTSLVKVLE FERDYMCNKC KHVFMVEADF EQYYTFSRPS SCPSLASCDS SKFSCLSDLS SSPARCRDYQ EIKIQEQVQR LSVGSIPRSM KVILEDDLVD SCKSGDDLTI YGVVMQRWKP FQRDVRCEVE IVLKANYVQV NNEQSSGMVM DEDTRKEFED FWEHYKSDPF AGRNEILASL CPQVFGMYLV KLAVAMVLAG GIQRTDAAGT RVRGESHLLL VGDPGTGKSQ FLKYAAKITP RSVLTTGIGS TSAGLTVTAV KDSGEWNLEA GALVLADAGL CCIDEFNSLK EHDRTSIHEA MEQQTISVAK AGLVCKLNTR TTILAATNPK GQYDPKESVS VNIALGSPLL SRFDLVLVLL DTRNEDWDRI ISSFILENKG YPSKSENLWS MEKMKTYFCL IRNLHPTLSE VSNQVLLRYY QMQRQSDSRN AARTTIRLLE SLIRLAEAHA RLMFRSAVTL EDAVTAVSVM ESSMQGGALL GGVNALHTSF PENPRAQYQR QCELILEKLE LQGLLQEELR RLERLQNESV HQCQSHSLEE EVAPGSCRND PRDKPRLRTS TQQEQSCSWS STERSGADSP PGPGLNRPTS CNNSAENRDG RGDGLDWLDP TSSPEIAPES TIVSPNVKTT EKNVNLKISN NKSQGKEKHG PQQRSKLLEA GHLPSSGAMN APLRSHGVKR TKASQAVVVS EAGRGDEEDS VPRRLPKLLK EGSQNVCRST TRVRPLPPTV PLSLSIPSPG SGKRSGTPKR KRRKSAQVEE PEPEGMETPT VKLAKFTFKQ KTKLTHSPEG QGPIPPSASE IAVDSSKIPQ QRTRREAAVP VVAPGKSTST SGDRCSDQLH GKTKELSRQP PDSNPPREER EQGPKRRVIQ PKPELGNQAG HSHLACEKDR KEGVSCGNKS SKVHAGTIAR LASFSFTSPS ESKSESLPPE RKDSRDSRDS RDSRDRCHSP PATTAPVLGQ QRQTFQLQQP TERANLSTLS LFTLSELDDE ALDFDWEEEM RKKP
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: MCM9 (Minichromosome Maintenance Deficient 9 (MCM9))

Andere Bezeichnung: Mcm9

Hintergrund: DNA helicase MCM9 (EC 3.6.4.12) (Mini-chromosome maintenance deficient domain-containing protein 1) (Minichromosome maintenance 9),FUNCTION: Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855, PubMed:22771120). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex at double-stranded DNA breaks to generate ssDNA by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (By similarity). Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs (PubMed:22771120, PubMed:23401855). Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand (PubMed:22771120, PubMed:26300262). In addition, recruits MLH1, a component of the MMR complex, to chromatin (By similarity). The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:21987787). Probably by regulating HR, plays a key role during gametogenesis (PubMed:21987787, PubMed:22771120). {ECO:0000250|UniProtKB:Q9NXL9, ECO:0000269|PubMed:21987787, ECO:0000269|PubMed:22771120, ECO:0000269|PubMed:23401855, ECO:0000269|PubMed:26300262}.

Molekulargewicht: 125.8 kDa

UniProt: Q2KHI9