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SULT2A2 Protein (AA 1-285) (His tag)

Crystallography grade SULT2A2 Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Produktnummer ABIN3134299
$9,343.16
Zzgl. Versandkosten $45.00 und $20.00 Trockeneis
Lieferung in 30 bis 35 Werktagen
  • Target
    SULT2A2 (Sulfotransferase Family 2A, Dehydroepiandrosterone (DHEA)-Preferring, Member 2 (SULT2A2))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-285
    Spezies
    Maus
    Quelle
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses SULT2A2 Protein ist gelabelt mit His tag.
    Applikation
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    MMSDYNWFEG IPFPAISYQR EILEDIRNKF VVKEEDLLIL TYPKSGTNWL NEIVCLIQTK GDPKWIQTVP IWDRSPWIET EIGYSAIINK EGPRLITSHL PIHLFSKSFF SSKAKAIYLM RNPRDILVSG YFFWGNTNLV KNPGSLGTYF EWFLQGNVLF GSWFEHVRGW LSMREWDNFL VLYYEDMKKD TKGTIKKICD FLGKNLGPDE LDLVLKYSSF QAMKENNMSN YSLIKEDRVT NGLKLMRKGT TGDWKNHFTV AQAEAFDKVF QEKMAGFPPG MFPWE
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Sult2a2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    SULT2A2 (Sulfotransferase Family 2A, Dehydroepiandrosterone (DHEA)-Preferring, Member 2 (SULT2A2))
    Andere Bezeichnung
    Sult2a2
    Synonyme
    C730007P19Rik Protein, ST Protein, ST2A2 Protein, Sth2 Protein, mSTa2 Protein, sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 2 Protein, Sult2a2 Protein
    Hintergrund
    Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze sulfonation of hydroxysteroids and xenobiotics. {ECO:0000250}.
    Molekulargewicht
    34.3 kDa Including tag.
    UniProt
    P50236
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