Pol (AA 1-1281) protein (Strep Tag)

Produktnummer ABIN3132845

Recombinant Pol-Protein exprimiert in Cell-free protein synthesis (CFPS).

Kurzübersicht für Pol (AA 1-1281) protein (Strep Tag) (ABIN3132845)

Target: Pol

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Cell-free protein synthesis (CFPS)

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Reinheit: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-1281

Aufreinigungstag / Konjugat: Strep Tag

Sequenz: MPTLTTKIKG SNNYFSLISL NINGLNSPIK RHRLTDWLHK QDPTFCCLQE THLREKDRHY LRVKGWKTIF QANGLKKQAG VAILISDKID FQPKVIKKDK EGHFILIKGK ILQEELSILN IYAPNARAAT FIRDTLVKLK AYIAPHTIIV GDFNTPLSSK DRSWKQKLNR DTVKLTEVMK QMDLTDIYRT FYPKTKGYTF FSAPHGTFSK IDHIIGHKTG LNRYKNIEIV PCILSDHHGL RLIFNNNINN GKPTFTWKLN NTLLNDTLVK EGIKKEIKDF LEFNENEATT YPNLWDTMKA FLRGKLIALS ASKKKRETAH TSSLTTHLKA LEKKEANSPK RSRRQEIIKL RGEINQVETR RTIQRINQTR SWFFEKINKI DKPLARLTKG HRDKILINKI RNEKGDITTD PEEIQNTIRS FYKRLYSTKL ENLDEMDKFL DRYQVPKLNQ DQVDHLNSPI SPKEIEAVIN SLPTKKSPGP DGFSAEFYQT FKEDLIPILH KLFHKIEVEG TLPNSFYEAT ITLIPKPQKD PTKIENFRPI SLMNIDAKIL NKILANRIQE HIKAIIHPDQ VGFIPGMQGW FNIRKSINVI HYINKLKDKN HMIISLDAEK AFDKIQHPFM IKVLERSGIQ GPYLNMIKAI YSKPVANIKV NGEKLEAIPL KSGTRQGCPL SPYLFNIVLE VLARAIRQQK EIKGIQIGKE EVKISLLADD MIVYISDPKN STRELLNLIN SFGEVVGYKI NSNKSMAFLY TKNKQAEKEI RETTPFSIVT NNIKYLGVTL TKEVKDLYDK NFKSLKKEIK EDLRRWKDLP CSWIGRINIV KMAILPKAIY RFNAIPIKIP TQFFNELEGA ICKFVWNNKK PRIAKSLLKD KRTSGGITMP DLKLYYRAIV IKTAWYWYRD RQVDQWNRIE DPEMNPHTYG HLIFDKGAKT IQWKKDSIFN NWCWHNWLLS CRRMRIDPYL SPCTKVKSKW IKELHIKPET LKLIEEKVGK SLEDMGTGEK FLNRTAMACA VRSRIDKWDL MKLQSFCKAK DTVNKTKRPP TDWERIFTYP KSDRGLISNI YKELKKVDFR KSNNPIKKWG SELNKEFSPE EYRMAEKHLK KCSTSLIIRE MQIKTTLRFH LTPVRMAKIK NSGDSRCWRG CGERGTLLHC WWECRLVQPL WKSVWRFLRK LDIVLPEDPA IPLLGIYPED APTGKKDTCS TMFIAALFII ARSWKEPRCP STEEWIQKMW YIYTMEYYSA IKKNEFMKFL AKWMDLEGII LSEVTHSQRN SHNMYSLISG Y
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: Pol

Substanzklasse: Viral Protein

Hintergrund: LINE-1 retrotransposable element ORF2 protein (ORF2p) (Long interspersed element-1) (L1) (Retrovirus-related Pol polyprotein LINE-1) [Includes: Reverse transcriptase (EC 2.7.7.49), Endonuclease (EC 3.1.21.-)],FUNCTION: Has reverse transcriptase activity required for target-primed reverse transcription of the LINE-1 element mRNA, a crucial step in LINE-1 retrotransposition. Also has endonuclease activity that allows the introduction of nicks in the chromosomal target DNA. Cleaves DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to sites of LINE-1 integration in the genome. Conformational properties of the target DNA sequence rather than specific nucleotides are key determinants of the ORF2p capacity for sequence-specific DNA recognition. Does not bend the DNA helix unlike related endonucleases but causes compression near the cleavage site. {ECO:0000250|UniProtKB:O00370}.

Molekulargewicht: 149.6 kDa

UniProt: P11369