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Aox3 Protein (AA 1-1335) (His tag)

Crystallography grade AOX3 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Produktnummer ABIN3131204
$24,004.89
Zzgl. Versandkosten $45.00 und $20.00 Trockeneis
Lieferung in 70 Werktagen
  • Target
    Aox3 (AOX3) (Aldehyde Oxidase 3 (AOX3))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1335
    Spezies
    Maus
    Quelle
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses Aox3 Protein ist gelabelt mit His tag.
    Applikation
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    MSPSKESDEL IFFVNGKKVT ERNADPEVNL LFYLRKVIRL TGTKYGCGGG DCGACTVMIS RYDPISKRIS HFSATACLVP ICSLHGAAVT TVEGIGSTKT RIHPVQERIA KGHGTQCGFC TPGMVMSIYT LLRNHPEPST EQIMETLGGN LCRCTGYRPI VESAKSFCPS STCCQMNGEG KCCLDEEKNE PERKNSVCTK LYEKKEFQPL DPTQELIFPP ELMRMAEESQ NTVLTFRGER TTWIAPGTLN DLLELKMKHP SAPLVIGNTY LGLHMKFTDV SYPIIISPAR ILELFVVTNT KQGLTLGAGL SLTQVKNVLS DVVSRLPKEK TQIYCALLKQ LKTLAGQQIR NVASLGGHII SRLPTSDLNP ILGIGNCILN VASTEGIQQI PLNDHFLAGV PDAILKPEQV LISVFVPRSS KWEFVSAFRQ APRQQNAFAT VNAGMKVVFK EDTNTITDLG ILYGGIGATV ISADKSCRQL IGRCWDEEML DDAGKMICEE VSLLMAAPGG MEEYRKTLAI SFLFMFYLDV LKQLKTRDPH KYPDISQKLL HILEDFPLTM PYGMQSFQDV DFQQPLQDPI GRPIMHQSGI KHATGEAVFC DDMSVLPGEL FLAVVTSSKS HAKIISLDAS EALASLGVVD VVTARDVPGD NGREEESLYA QDEVICVGQI VCAVAADSYA HAQQAAKKVK IVYQDIEPMI VTVQDALQYE SFIGPERKLE QGNVEEAFQC ADQILEGEVH LGGQEHFYME TQSVRVVPKG EDKEMDIYVS SQDAAFTQEM VARTLGIPKN RINCHVKRVG GAFGGKASKP GLLASVAAVA AQKTGRPIRF ILERRDDMLI TGGRHPLLGK YKIGFMNNGK IKAADIQLYI NGGCTPDDSE LVIEYALLKL ENAYKIPNLR VRGRVCKTNL PSNTAFRGFG FPQGAFVTET CMSAVAAKCR LPPEKVRELN MYRTIDRTIH NQEFDPTNLL QCWEACVENS SYYNRKKAVD EFNQQRFWKK RGIAIIPMKF SVGFPKTFYY QAAALVQIYT DGSVLVAHGG VELGQGINTK MIQVASRELK IPMSYIHLDE MSTVTVPNTV TTGASTGADV NGRAVQNACQ ILMKRLEPII KQNPSGTWEE WVKEAFVQSI SLSATGYFRG YQADMDWEKG EGDIFPYFVF GAACSEVEID CLTGAHKNIR TDIVMDGSFS INPAVDIGQI EGAFVQGLGL YTLEELKYSP EGVLYTRGPH QYKIASVTDI PEEFHVSLLT PTPNPKAIYS SKGLGEAGTF LGCSVFFAIA AAVAAAREER GLSPIWAINS PATAEVIRMA CEDQFTNLVP QTDSKCCKPW SIPVA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Aox3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    Aox3 (AOX3) (Aldehyde Oxidase 3 (AOX3))
    Andere Bezeichnung
    Aox3
    Synonyme
    Aoh1 Protein, Aoh2 Protein, AI326299 Protein, 1200011D03Rik Protein, aldehyde oxidase 3 Protein, Aox3 Protein
    Hintergrund
    Oxidase with broad substrate specificity, oxidizing aromatic azaheterocycles, such as N1-methylnicotinamide and phthalazine, as well as aldehydes, such as benzaldehyde, retinal and pyridoxal. Plays a key role in the metabolism of xenobiotics and drugs containing aromatic azaheterocyclic substituents. Is probably involved in the regulation of reactive oxygen species homeostasis. May be a prominent source of superoxide generation via the one-electron reduction of molecular oxygen. Also may catalyze nitric oxide (NO) production via the reduction of nitrite to NO with NADH or aldehyde as electron donor. {ECO:0000269|PubMed:11562361, ECO:0000269|PubMed:18981221, ECO:0000269|PubMed:21705476, ECO:0000269|PubMed:23019336}.
    Molekulargewicht
    147.9 kDa Including tag.
    UniProt
    G3X982
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