HEYL Protein (AA 1-326) (Strep Tag)

Produktnummer ABIN3129607

Dieses Recombinant HEYL-Protein wird in Cell-free protein synthesis (CFPS) exprimiert.

Kurzübersicht für HEYL Protein (AA 1-326) (Strep Tag) (ABIN3129607)

Target: HEYL (Hairy/enhancer-of-Split Related with YRPW Motif-Like (HEYL))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Cell-free protein synthesis (CFPS)

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Reinheit: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-326

Aufreinigungstag / Konjugat: Dieses HEYL Protein ist gelabelt mit Strep Tag.

Sequenz: MKRPRAPSGS DGESDGPIDV GQENDLSQMA RPLTTPSPSQ MQARKKRRGI IEKRRRDRIN SSLSELRRLV PTAFEKQGSS KLEKAEVLQM TVDHLKMLHA SGGTGFFDAR ALAVDFRSIG FRECLTEVIR YLGVLEGPSS HADPVRIRLL SHLKSYAAEM EPSPTTTSAL AFPVWPWSFL HSCPGLPSLN SQLAILGRVP GPVLPSISSP PYPISALRSA PVHRPAGTIP PTRRNLLPSR GVTSTQRAHL PERPAAPPPT ALDARAARSI VPIPPCSPTT APGAGKSDDN VSGSISSPCP SGPTGRPAGA VFYHSWVSEI TEIGAF
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: HEYL (Hairy/enhancer-of-Split Related with YRPW Motif-Like (HEYL))

Andere Bezeichnung: Heyl

Hintergrund: Hairy/enhancer-of-split related with YRPW motif-like protein (Hairy and enhancer of split-related protein 3) (Hairy-related transcription factor 3) (HRT-3) (mHRT3),FUNCTION: Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Downstream effector of Notch signaling required for cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6. {ECO:0000250, ECO:0000269|PubMed:16199874, ECO:0000269|PubMed:17303760}.

Molekulargewicht: 34.9 kDa

UniProt: Q9DBX7

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling