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PUS10 Protein (AA 1-527) (Strep Tag)

PUS10 Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Produktnummer ABIN3129028
  • Target Alle PUS10 Proteine anzeigen
    PUS10 (Pseudouridylate Synthase 10 (PUS10))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-527
    Spezies
    Maus
    Quelle
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Aufreinigungstag / Konjugat
    Dieses PUS10 Protein ist gelabelt mit Strep Tag.
    Applikation
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    MLPLTEENKH VAQLLFSSGT CPRCILRFCG VDLPAPYKHP SKELLNELQK FLEPEKPELI LEAPNPPLKK IRLHEDGIDN LSEDGKEGVS VTEDESMAEK PSKLRVCNVC LGILQEFCEK GFITKVCQKV EASGFEFTSV VLSVSFPPQL SVREHAAWLL VKQEMGKQSL SLGRNDVVQL KEAYKWITHP LFSEELGVPT DGKSLFEVSV VFAHPETAED CHFLGEVCRD CFKPAKNKQS VFTRMAVLKA LSKIKEEDFL GQFPCPPNSP KTVCTVLEVE CTHGAVFVAG RYNKYSRNLP QTPWIIDGER KMESSVEELI SDHLLAVFRA ESFNFSSSGR EDVDVRTLGN GRPFAVELLN PHRVHFTSQE MKELQQTINK SSDKIQVRDL QLVTREAIGH MKEGEEEKTK TYSALIWTNR AIQKKDIGFL DDLKDLKIDQ KTPLRVLHRR PLAVRTRAIH SMKTHYLDEH HFRLHLKTQA GTYIKEFVHG DFGRTKPNLG SLMNVTADIL ELDVESVDVD WPPALDD
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Produktmerkmale
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Endotoxin-Niveau
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    Top Product
    Discover our top product PUS10 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    PUS10 (Pseudouridylate Synthase 10 (PUS10))
    Andere Bezeichnung
    Pus10 (PUS10 Produkte)
    Synonyme
    CCDC139 Protein, DOBI Protein, 2810013G11Rik Protein, 4933435A13Rik Protein, AU014648 Protein, C77560 Protein, Ccdc139 Protein, RGD1306402 Protein, pseudouridylate synthase 10 Protein, PUS10 Protein, Pus10 Protein
    Hintergrund
    TRNA pseudouridine synthase Pus10 (EC 5.4.99.25) (Coiled-coil domain-containing protein 139) (tRNA pseudouridine 55 synthase) (Psi55 synthase) (tRNA pseudouridylate synthase) (tRNA-uridine isomerase),FUNCTION: Protein with different functions depending on its subcellular location: involved in miRNA processing in the nucleus and acts as a tRNA pseudouridylate synthase in the cytoplasm. In the cytoplasm, acts as a pseudouridylate synthase by catalyzing synthesis of pseudouridine(54) and pseudouridine(55) from uracil-54 and uracil-55, respectively, in the psi GC loop of a subset of tRNAs. tRNA pseudouridylate synthase activity is enhanced by the presence of 1-methyladenosine at position 53-61 of tRNAs. Does not show tRNA pseudouridylate synthase activity in the nucleus. In the nucleus, promotes primary microRNAs (pri-miRNAs) processing independently of its RNA pseudouridylate synthase activity. Binds pri-miRNAs. Modulator of TRAIL/TNFSF10-induced cell death via activation of procaspase-8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death. {ECO:0000250|UniProtKB:Q3MIT2}.
    Molekulargewicht
    59.7 kDa
    UniProt
    Q9D3U0
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