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LRRC50 Protein (AA 1-634) (Strep Tag)

LRRC50 Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Produktnummer ABIN3128949
  • Target Alle LRRC50 Proteine anzeigen
    LRRC50 (Leucine Rich Repeat Containing 50 (LRRC50))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-634
    Spezies
    Maus
    Quelle
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Aufreinigungstag / Konjugat
    Dieses LRRC50 Protein ist gelabelt mit Strep Tag.
    Applikation
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    MHPEVSEPPV DSVAEPSLEE SAGDHGDAGP GIRKEEISET KETCAGPCTT SCPSQQQPSG DNGSEGFCTH SRDDREDRGP RMTKQFLQKL CKQHKLYVTP ALNDTLYLHF KGFDRIENLE EYTGLRCLWL ECNGIQRIEN LQAQSELRCL FLQVNLLHKI ENLEPLQKLD ALNLSNNYIK TIENLSCLPV LNTLQMAHNR LETVADIEHL RECLRLCVLD LSHNALSDPE ILSVLESMPC LRVLNLMGNP VTKHIPNYRR TVTVRLKHLT YLDDRPVFPK DRACAEAWAR GGYAAEKEER RQWESREHKK ITDSLEALAM IKRRAEERKK ARDRGETPLP DSEGSIPTSP EAEEKQPMGE IQKKMELFVE ESFEAKDELF PETPGGEKEL HVVVVNGAVE NPDLSGSLAH NQTPVVVTPE ESTSPVAATD GARTEDIEAV AVEIKERLFI DDLPDLEDAE GTDVSVEDQI KETDIPRIQA ISSLSDDSDP ELDELSLSTS EATPTGATGA LSHIFAISKG PSTAATVPFT DICKPIATTD LESQGQDCGA AASRPLIQEL NDEPAEEAAN QPLPPQTCAS DPALAHPSED GDSDSQLPAA TLLGDGAEDE AESSVHPKEP STRVGLEDIE FGLD
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Produktmerkmale
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Endotoxin-Niveau
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    Top Product
    Discover our top product LRRC50 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    LRRC50 (Leucine Rich Repeat Containing 50 (LRRC50))
    Andere Bezeichnung
    Dnaaf1 (LRRC50 Produkte)
    Synonyme
    4930457P18Rik Protein, Lrrc50 Protein, LRRC50 Protein, CILD13 Protein, ODA7 Protein, LLRC50 Protein, RGD1310542 Protein, lrrc50 Protein, zgc:56169 Protein, dynein, axonemal assembly factor 1 Protein, dynein axonemal assembly factor 1 Protein, dynein, axonemal, assembly factor 1 Protein, Dnaaf1 Protein, DNAAF1 Protein, dnaaf1 Protein
    Hintergrund
    Dynein axonemal assembly factor 1 (Leucine-rich repeat-containing protein 50),FUNCTION: Cilium-specific protein required for the stability of the ciliary architecture. Plays a role in cytoplasmic preassembly of dynein arms (By similarity). Involved in regulation of microtubule-based cilia and actin-based brush border microvilli (By similarity). {ECO:0000250}.
    Molekulargewicht
    69.7 kDa
    UniProt
    Q9D2H9
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