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TRAPPC6B Protein (AA 1-158) (Strep Tag)

TRAPPC6B Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Produktnummer ABIN3128930
  • Target Alle TRAPPC6B Proteine anzeigen
    TRAPPC6B (Trafficking Protein Particle Complex 6B (TRAPPC6B))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-158
    Spezies
    • 1
    • 1
    Maus
    Quelle
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Aufreinigungstag / Konjugat
    Dieses TRAPPC6B Protein ist gelabelt mit Strep Tag.
    Applikation
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    Sequenz
    MADEALFLLL HNEMVSGVYK SAEQGEVENG RCVTKLESMG FRVGQGLIER FTKDTARFKD ELDIMKFICK DFWTTVFKKQ IDNLRTNHQG IYVLQDNKFR LLIQLSAGKQ YLEHASKYLA FTCGLIRGGL SNLGIKSIVT AEVSSMPACK FQVMIQKL
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Produktmerkmale
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Endotoxin-Niveau
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    Top Product
    Discover our top product TRAPPC6B Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    TRAPPC6B (Trafficking Protein Particle Complex 6B (TRAPPC6B))
    Andere Bezeichnung
    Trappc6b (TRAPPC6B Produkte)
    Synonyme
    zgc:103464 Protein, TPC6 Protein, 5830498C14Rik Protein, C79212 Protein, RGD1309325 Protein, trafficking protein particle complex 6b Protein, Trafficking protein particle complex subunit 6B Protein, trafficking protein particle complex subunit 6B Protein, trafficking protein particle complex subunit 6b Protein, trafficking protein particle complex 6B Protein, trafficking protein particle complex 6B L homeolog Protein, LOC733029 Protein, cgd3_2700 Protein, NCU06212 Protein, ATEG_05652 Protein, LOC5563708 Protein, CC1G_04333 Protein, CpipJ_CPIJ005932 Protein, PTRG_01279 Protein, TTHERM_00657360 Protein, PITG_00953 Protein, Tsp_08307 Protein, trappc6b Protein, trappc6b.L Protein, TRAPPC6B Protein, Trappc6b Protein
    Hintergrund
    Trafficking protein particle complex subunit 6B (TRAPP complex subunit 6B),FUNCTION: Component of a transport protein particle (TRAPP) complex that may function in specific stages of inter-organelle traffic (By similarity). Specifically involved in the early development of neural circuitry, likely by controlling the frequency and amplitude of intracellular calcium transients implicated in the regulation of neuron differentiation and survival (By similarity). {ECO:0000250|UniProtKB:Q86SZ2}.
    Molekulargewicht
    17.9 kDa
    UniProt
    Q9D289
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