Serine (Or Cysteine) Peptidase Inhibitor, Clade B, Member 1a (SERPINB1A) (AA 1-379) protein (Strep Tag)

Produktnummer ABIN3128850

Produktdetails

Target: Serine (Or Cysteine) Peptidase Inhibitor, Clade B, Member 1a (SERPINB1A)

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-379

Spezies: Maus

Quelle: Cell-free protein synthesis (CFPS)

Aufreinigungstag / Konjugat: Strep Tag

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Marke: AliCE®

Sequenz: MEQLSSANTL FALELFQTLN ESSPTGNIFF SPFSISSALA MVILGAKGST AAQLSKTFHF DSVEDIHSRF QSLNAEVSKR GASHTLKLAN RLYGEKTYNF LPEYLASTQK MYGADLAPVD FLHASEDARK EINQWVKGQT EGKIPELLSV GVVDSMTKLV LVNAIYFKGM WEEKFMTEDT TDAPFRLSKK DTKTVKMMYQ KKKFPFGYIS DLKCKVLEMP YQGGELSMVI LLPKDIEDES TGLKKIEKQI TLEKLLEWTK RENLEFIDVH VKLPRFKIEE SYTLNSNLGR LGVQDLFSSS KADLSGMSGS RDLFISKIVH KSFVEVNEEG TEAAAATGGI ATFCMLLPEE EFTVDHPFIF FIRHNPTSNV LFLGRVCSP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Reinheit: > 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse: custom-made

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: Serine (Or Cysteine) Peptidase Inhibitor, Clade B, Member 1a (SERPINB1A)

Andere Bezeichnung: Serpinb1a (SERPINB1A Produkte)

Hintergrund: Leukocyte elastase inhibitor A (Serine protease inhibitor EIA) (Serpin B1a),FUNCTION: Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis (PubMed:17664292, PubMed:21683252, PubMed:21248149, PubMed:30692621). Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection (PubMed:17664292). These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Acts also as a potent intracellular inhibitor of granzyme H (PubMed:12189154). During inflammation, limits the activity of inflammatory caspases CASP1 and CASP4 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation (PubMed:30692621). In addition, promotes the proliferation of beta-cells when secreted (PubMed:26701651). {ECO:0000269|PubMed:12189154, ECO:0000269|PubMed:17664292, ECO:0000269|PubMed:21248149, ECO:0000269|PubMed:21683252, ECO:0000269|PubMed:26701651, ECO:0000269|PubMed:30692621}.

Molekulargewicht: 42.6 kDa

UniProt: Q9D154