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CRYBG3 Protein (AA 1-1005) (His tag)

Crystallography grade CRYBG3 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Produktnummer ABIN3124351
$24,004.89
Zzgl. Versandkosten $45.00 und $20.00 Trockeneis
Lieferung in 70 Werktagen
  • Target
    CRYBG3 (beta-gamma Crystallin Domain Containing 3 (CRYBG3))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1005
    Spezies
    Maus
    Quelle
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses CRYBG3 Protein ist gelabelt mit His tag.
    Applikation
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    MSVPVVYDKR RNLDCREEKE ESNLAFVSQD EQDSSSFTIL YEEPLQEEDR YTSAELRGSQ SLLFPDTSSM PGLACERSES RTDLVHHFEK EGKLGEAFDG DNSEMFLSVE AKRYKIYPLA LSPIYEDDSS QEDVLSSEVS PGHHGSSKSR ESANQPSSVL SLLQSVSERL QRNFDGDDRQ EAEEEEEEAV ASGKDWRTEK REHVTFHLPD PSIPFYPEDN QEHAGIFKSY VEFSEPTTSS LQHGRWSEKE LFLQKSDMTS KLHSSLKSAY HQYLQTSRTH SSETGTRFGG TLQEPVSKYF RVQDHAGRLS PYVENVDKQT LKCNPRPGKM IIYDLHGSKY KQEVYCNIPD ATTWSFPNGA LIKVVRGCWI LYEKPHFQGQ KCVLEEGERV LDRDWLLQNR KHPERNFVLG SIKRVLKDCS IPVIELCPKT DPGCSPIYIH RSVPNVEELN IPKSTSVTVK SGVWLAYPDI HFKGQATILE EDQGLFEISA AEMKSLHPLQ MGGLKVEMPM NLKVILYEKP HFLGHTKEFS EHIDSVPTFL KSDKDFHGIG SIRVIGGVWV AYEKEHFKGQ QFLLEEGDFE DSSACGALSG PIMSFRYLQA NFIESSITLF ESSHLESGKF IDITNQEISD LEEIGFGSET RSIHVKSGVW VAYHQKFFCG DQYILEKGKY KCFFDWGGSS NTILSIRPIQ LEPLGINEPT HLLKAFSKAG FQGECIDFVK ECADLTSFTP ASFKVLRGCW LLLYYQEDGF YHQCVLEEGL YVDLTSCGCP SARIRALQPI DYVFEEPSIS LFALEHCEGR ELHLEDAVNS VLNKDLHFYT QSVWIKSGLW IAYEGSNFLG RQILLTPKEI PNWTAFSGWK TIGSVRPMKQ PAVYIRIRNR AQDEYLTVTG NPADARTMSV CISPYSGKDT QIWHYCRGLF KSKASHTCLD VIGGRDTPGA KVALWTEHGQ LRQKWRMSRN GTISSYLSDE LVLDVKGGNY YDKTHVIVNQ PLEGEETQKW DIEIL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Crybg3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    CRYBG3 (beta-gamma Crystallin Domain Containing 3 (CRYBG3))
    Andere Bezeichnung
    Crybg3
    Synonyme
    DKFZp667G2110 Protein, 5031404N19 Protein, AU022790 Protein, crystallin beta-gamma domain containing 3 Protein, beta-gamma crystallin domain containing 3 Protein, CRYBG3 Protein, Crybg3 Protein
    Molekulargewicht
    115.2 kDa Including tag.
    UniProt
    Q80W49
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