KLHL13 Protein (AA 1-654) (Strep Tag)

Produktnummer ABIN3124249

Dieses Recombinant KLHL13-Protein wird in Cell-free protein synthesis (CFPS) exprimiert.

Kurzübersicht für KLHL13 Protein (AA 1-654) (Strep Tag) (ABIN3124249)

Target: KLHL13 (Kelch-Like 13 (KLHL13))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Cell-free protein synthesis (CFPS)

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Reinheit: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-654

Aufreinigungstag / Konjugat: Dieses KLHL13 Protein ist gelabelt mit Strep Tag.

Sequenz: MPLKWKTSSP AIWKFPVPVL KTSRSTPLSP AYISLVEEED QHLKLCLGSE MGLSSHLQSC KAGSTRIFTS NSHSSVVLQG FDQLRLDGLL CDVTLMPGDT DDAYPVHRVM MASASDYFKA MFTGGMKEQE LMCIKLHGVS RVGLRKIIDF IYTAKLSLNM DTLQDTLEAA SFLQILPVLD FCKVFLISGV TLDNCVEVGR IANTYHLTEV DKYVNSFVLK NFAALLSTGE FLKLPFERLA FVLSSNSLKR CTELDLFKAT CRWLRLEEPR MDVAAKLMKN IRFPLMTPQE LINYVQTVDF MRTDNTCVNL LLEASNYQMM PFMQPVMQSD RTAIRSDTTR LVTLGGVLRQ QLVVSKELRM YDEKTHEWKS LAPMDAPRYQ HGIAVIGNFL YVVGGQSNYD TKGKTAVDTV FRFDPRYNKW IQVASLNEKR TFFHLSALKG FLYAVGGRNA AGELPTVECY NPRTNEWTYV AKMNEPHYGH AGTVYGGVMY ISGGITHDTF QKELMCFDPD TDKWTQKAPM TTVRGLHCMC TVGDRLYVIG GNHFRGTSDY DDVLSCEYYS PILDQWTPIA SMLRGQSDVG VAVFENKIYV VGGYSWNNRC MVEIVQKYDP EKNEWHKVFD LPESLGGIRA CTLTVYPPEE ATPSPSRESP LSGP
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: KLHL13 (Kelch-Like 13 (KLHL13))

Andere Bezeichnung: Klhl13

Hintergrund: Kelch-like protein 13 (BTB and kelch domain-containing protein 2),FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis (By similarity). {ECO:0000250}.

Molekulargewicht: 73.8 kDa

UniProt: Q80TF4