ZNF93 Protein (AA 1-620) (Strep Tag)

Produktnummer ABIN3096451

Dieses Recombinant ZNF93-Protein wird in Cell-free protein synthesis (CFPS) produziert.

Kurzübersicht für ZNF93 Protein (AA 1-620) (Strep Tag) (ABIN3096451)

Target: ZNF93 (Zinc Finger Protein 93 (ZNF93))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Cell-free protein synthesis (CFPS)

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Reinheit: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-620

Aufreinigungstag / Konjugat: Dieses ZNF93 Protein ist gelabelt mit Strep Tag.

Sequenz: MGPLQFRDVA IEFSLEEWHC LDTAQRNLYR NVMLENYSNL VFLGIVVSKP DLIAHLEQGK KPLTMKRHEM VANPSVICSH FAQDLWPEQN IKDSFQKVIL RRYEKRGHGN LQLIKRCESV DECKVHTGGY NGLNQCSTTT QSKVFQCDKY GKVFHKFSNS NRHNIRHTEK KPFKCIECGK AFNQFSTLIT HKKIHTGEKP YICEECGKAF KYSSALNTHK RIHTGEKPYK CDKCDKAFIA SSTLSKHEII HTGKKPYKCE ECGKAFNQSS TLTKHKKIHT GEKPYKCEEC GKAFNQSSTL TKHKKIHTGE KPYVCEECGK AFKYSRILTT HKRIHTGEKP YKCNKCGKAF IASSTLSRHE FIHMGKKHYK CEECGKAFIW SSVLTRHKRV HTGEKPYKCE ECGKAFKYSS TLSSHKRSHT GEKPYKCEEC GKAFVASSTL SKHEIIHTGK KPYKCEECGK AFNQSSSLTK HKKIHTGEKP YKCEECGKAF NQSSSLTKHK KIHTGEKPYK CEECGKAFNQ SSTLIKHKKI HTREKPYKCE ECGKAFHLST HLTTHKILHT GEKPYRCREC GKAFNHSATL SSHKKIHSGE KPYECDKCGK AFISPSSLSR HEIIHTGEKP
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: ZNF93 (Zinc Finger Protein 93 (ZNF93))

Andere Bezeichnung: ZNF93

Hintergrund: Zinc finger protein 93 (Zinc finger protein 505) (Zinc finger protein HTF34),FUNCTION: Transcription factor specifically required to repress long interspersed nuclear element 1 (L1) retrotransposons: recognizes and binds L1 sequences and repress their expression by recruiting a repressive complex containing TRIM28/KAP1 (PubMed:25274305). Not able to repress expression of all subtypes of L1 elements. Binds to the 5' end of L1PA4, L1PA5 and L1PA6 subtypes, and some L1PA3 subtypes. Does not bind to L1PA7 or older subtypes nor at the most recently evolved L1PA2 and L1Hs. 50 % of L1PA3 elements have lost the ZNF93-binding site, explaining why ZNF93 is not able to repress their expression (PubMed:25274305). {ECO:0000269|PubMed:25274305}.

Molekulargewicht: 71.0 kDa

UniProt: P35789