Gm1564 (AA 1-952) protein (Strep Tag)

Produktnummer ABIN3093770

Dieses Recombinant Gm1564-Protein wird in Cell-free protein synthesis (CFPS) produziert.

Kurzübersicht für Gm1564 (AA 1-952) protein (Strep Tag) (ABIN3093770)

Target: Gm1564

Protein-Typ: Recombinant

Spezies: Human

Quelle: Cell-free protein synthesis (CFPS)

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Reinheit: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-952

Aufreinigungstag / Konjugat: Strep Tag

Sequenz: MEVRRGDTCP RPHPSGLREE GLEPKVAFPG GANRCWNLGA DAGSRLTDVF GSVMLTGSAS FYDCYTSQSE DNVDLRQTYT PFSSTEYSSS VDSSLFCAPW STYGDDIKQP SNSQISIKNR IQTERNDYGS ETDLYGLVSN ILEEQDKSQP YFAEGTCSSN LKSVWPMNTS RFADHHDLLT ETKRPIDTVI SQQAFYSDES VSAMEKQYLR NSNLTPQQKI DELHHGFTGL DLEEQWMYPS RSDHSNCHNI QTNDTAKTTF QEYPLIKNCF TPQTGLSDIM KESGVDIYHY GRDRICTKGL EAPLQQKRAE MFLSQFNRYN ENVDYCRYPE YVHPNKAKLN KCSNFSVQDS KKLANGTPET PTVEADTYTK LFQVKPANQK KMEETIPDQQ NFTFPKTTPH LTEKQFAKEA VFTADFGLTS EYGLKPHTAC PANDFANVTE KQQFAKPDPP HSEYFKSVNL LSNSATSSGG INLNRPTWMN VQTKNNTPIP YRNQGNLMKL NSHLSAASKG SNHSSDFPQL SSTNLTPNSN LFQKYCQENP SAFSSFDFSY SGAERIQSVN HIEGLTKPGE ENLFKLVTDK KIKQPNGFCD NYSAQKYGII ENVNKHNFQA KPQSGHYDPE EGPKHLDGLS QNTYQDLLES QGHSNSHRTR GGDNSRVNRT QVSCFSNNYM MGDLRHNQCF QQLGSNGFPL RSTHPFGHSV VPLLDSYDLL SYDDLSHLYP YFNMMYGDNS FSGLMPTFGF QRPIKTRSGP ASELHIRLEE CCEQWRALEK ERKKTELALA KNYPGKKVSS TNNTPVPRLT SNPSRVDRLI VDELRELARV VTLLGKMERL RSSLLHASIS TALDRHLESI HIVQSRRKDE IVNASNRQRQ GVPRCQDDRD VFALASAIKE MCVATRKTRT ALWCALQMTL PKTASTADVV KPLQDTVNCE DKVHESINSS NPMNQRGETN KH
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: Gm1564

Andere Bezeichnung: MEIOC

Hintergrund: Meiosis-specific coiled-coil domain-containing protein MEIOC (Meiosis-specific with coiled-coil domain protein),FUNCTION: Is required for meiosis completion in both male and female germ cells. Confers stability to numerous meiotic mRNAs in gonads allowing proper initiation and progression into meiosis prophase I. The function may involve YTHDC2 and is independent of induction by retinoic acid (RA). Maintains an extended meiotic prophase I by properly promoting the transition from a mitotic to a meiotic cell cycle program by binding transcripts through its interaction with YTHDC2 that regulate the mitotic cell cycle. {ECO:0000250|UniProtKB:A2AG06}.

Molekulargewicht: 107.6 kDa

UniProt: A2RUB1