Gap Junction Protein, gamma 2, 47kDa (GJC2) (AA 1-439) protein (rho-1D4 tag)

Details zu Produkt Nr. ABIN3078600
Proteinname
  • GJA12
  • cx47
  • gja12
  • cx46.6
  • pmldar
  • MGC146420
  • B230382L12Rik
  • Cx47
  • Gja12
  • CX46.6
  • HLD2
  • LMPH1C
  • PMLDAR
  • SPG44
  • gap junction protein gamma 2
  • si:dkey-91f15.1
  • gap junction protein, gamma 2
  • GJC2
  • gjc2
  • si:dkey-91f15.1
  • Gjc2
Proteineigenschaft
AA 1-439
1
1
1
1
1
Spezies
Human
3
2
Quelle
Insektenzellen
5
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
rho-1D4 tag
Applikation
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Sequenz MTNMSWSFLT RLLEEIHNHS TFVGKVWLTV LVVFRIVLTA VGGEAIYSDE QAKFTCNTRQ PGCDNVCYDA FAPLSHVRFW VFQIVVISTP SVMYLGYAVH RLARASEQER RRALRRRPGP RRAPRAHLPP PHAGWPEPAD LGEEEPMLGL GEEEEEEETG AAEGAGEEAE EAGAEEACTK AVGADGKAAG TPGPTGQHDG RRRIQREGLM RVYVAQLVAR AAFEVAFLVG QYLLYGFEVR PFFPCSRQPC PHVVDCFVSR PTEKTVFLLV MYVVSCLCLL LNLCEMAHLG LGSAQDAVRG RRGPPASAPA PAPRPPPCAF PAAAAGLACP PDYSLVVRAA ERARAHDQNL ANLALQALRD GAAAGDRDRD SSPCVGLPAA SRGPPRAGAP ASRTGSATSA GTVGEQGRPG THERPGAKPR AGSEKGSASS RDGKTTVWIGSSGTETSQVAPA
C-terminal Rho-tag
Produktmerkmale
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human GJC2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

Reinigung The protein is purified from the cleared cell lysate using Rho1D4 capture materials. Eluate fractions are analyzed by SDS-PAGE.
Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Reinheit >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterilität 0.22 μm filtered
Endotoxin-Niveau Protein is endotoxin-free.
Güteklasse Crystallography grade
Proteinname
Hintergrund One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a role in myelination in central and peripheral nervous systems. {ECO:0000269|PubMed:15192806}.
Molekulargewicht 48.2 kDa Including tag.
UniProt Q5T442
Applikations-hinweise In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer in solution (20 mM Hepes, pH 7.4, 100 mM NaCl, 0,1 % LDAO)
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -80 °C
Informationen zur Lagerung Store at -80°C.
Haltbarkeit Unlimited (if stored properly)
Bilder des Herstellers
 image for Gap Junction Protein, gamma 2, 47kDa (GJC2) (AA 1-439) protein (rho-1D4 tag) (ABIN3078600) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
SDS-PAGE (SDS) image for Gap Junction Protein, gamma 2, 47kDa (GJC2) (AA 1-439) protein (rho-1D4 tag) (ABIN3078600) Gap junction gamma-2 protein (GJC2) (AA 1-439), fraction 9 - 10
Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC) image for Gap Junction Protein, gamma 2, 47kDa (GJC2) (AA 1-439) protein (rho-1D4 tag) (ABIN3078600) Gap junction gamma-2 protein (GJC2) (AA 1-439), gel filtration, Superose 6 fraction 9...
Western Blotting (WB) image for Gap Junction Protein, gamma 2, 47kDa (GJC2) (AA 1-439) protein (rho-1D4 tag) (ABIN3078600) Gap junction gamma-2 protein (GJC2) (AA 1-439), fraction 9 - 10
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