Fc Fragment of IgG, Receptor, Transporter, alpha (FCGRT) (Active) protein (Strep Tag,AVI tag,Biotin,His tag)

Details zu Produkt Nr. ABIN2870582, Anbieter: Anmelden zum Anzeigen
  • FCRN
  • alpha-chain
  • FcRn
  • Fc fragment of IgG receptor and transporter
  • Fc receptor, IgG, alpha chain transporter
  • Fcgrt
Ratte (Rattus)
HEK-293 Cells
Biologische Aktivität
Aufreinigungstag / Konjugat
Dieses FCGRT Protein ist gelabelt mit Strep Tag,AVI tag,Biotin,His tag.
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Hersteller Produkt- Nr.
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Marke MABSol®,PrecisionAvi
Spezifität Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Produktmerkmale The subunit FCGRT carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The subunit Beta-2 microglobulin (B2M) carries a Strep II tag at the C-terminus. The protein has a calculated MW of 34.7 kDa (FCGRT), 13.1 kDa (B2M) . The protein migrates as 45-50 kDa (FCGRT), 14 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Reinheit >95 % as determined by SDS-PAGE.
Endotoxin-Niveau Less than 1.0 EU per μg by the LAL method.
Hintergrund FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
Molekulargewicht 34.7 kDa (FCGRT), 13.1 kDa (B2M)
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Lagerung -20 °C
Bilder des Herstellers
SDS-PAGE (SDS) image for Fc Fragment of IgG, Receptor, Transporter, alpha (FCGRT) (Active) protein (Strep Tag,AVI tag,Biotin,His tag) (ABIN2870582) Biotinylated Rat FcRn / FCGRT & B2M on SDS-PAGE under reducing (R) condition. The gel...
Binding Studies (Bind) image for Fc Fragment of IgG, Receptor, Transporter, alpha (FCGRT) (Active) protein (Strep Tag,AVI tag,Biotin,His tag) (ABIN2870582) Immobilized Yervoy Ipilimumab at 5 μg/mL (100 μL/well) can bind Biotinylated Rat FcRn...
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