VEGF 165 (Active) Protein

Produktnummer ABIN2746009

Dieses Recombinant -Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für VEGF 165 (Active) Protein (ABIN2746009)

Target: VEGF 165

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: >95 % (SDS-PAGE)

Produktdetails

Verwendungszweck: VEGF 165 (human) (rec.)

Kreuzreaktivität: Human

Produktmerkmale: Human VEGF 165 (165aa).

Endotoxin-Niveau: <0.1ng/μg protein.

Biological Activity Comment: The ED50 for stimulation of cell proliferation in human umbilical vein endothelial cells by VEGF 165 has been determined to be in the range of 1-5ng/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 mM acetic acid to a concentration not lower than 50 μg/mL.

Handhabung: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. For long term storage we recommend to add at least 0.1 % human or bovine serum albumin.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.

Haltbarkeit: 6 months

Antigendetails

Target: VEGF 165

Hintergrund:

Vascular Endothelial Growth Factor, VEGF-A, VPF

Human vascular endothelial growth factor (VEGF 165) is produced as a homodimer. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. VEGF 165 is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. It is also a chemoattractant molecule for monocytes and endothelial cells. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels.

Molekulargewicht: ~38.2kDa

UniProt: P15692-4