Profilin Protein (PFN) (AA 1-163)

Produktnummer ABIN2745816

Produktdetails

Target: Profilin (PFN)

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-163

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Verwendungszweck: Profilin (Toxoplasma gondii) (rec.)

Kreuzreaktivität: Maus

Produktmerkmale: Recombinant profilin (Toxoplasma gondii) (aa 1-163).

Reinheit: >95 % (SDS-PAGE)

Endotoxin-Niveau: <0.01EU/μg purified protein (LAL test).

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 100 μL sterile water.

Konzentration: 0.1 mg/mL

Buffer: Contains PBS.

Handhabung: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Haltbarkeit: 6 months

Antigendetails

Target: Profilin (PFN)

Andere Bezeichnung: Profilin (PFN Produkte)

Hintergrund:

Inflammatory Profilin

TLR11, an innate sensor for profilin (Toxoplasma gondii), is an intracellular receptor that resides in the endoplasmic reticulum. The 12 membrane-spanning endoplasmic reticulum-resident protein UNC93B1 interacts directly with TLR11 and regulates the activation of dendritic cells in response to profilin (Toxoplasma gondii) and parasitic infection in vivo. TLR12, a previously uncharacterized TLR, also recognizes profilin from Toxoplasma gondii. TLR12 is sufficient for recognition of profilin by plasmacytoid dendritic cells (pDCs), whereas TLR11 and TLR12 are both required in macrophages and conventional DCs. In contrast to TLR11, TLR12-deficient mice succumb rapidly to T. gondii infection.

Molekulargewicht: ~20kDa (SDS-PAGE)

UniProt: Q58NA1