NAPRT1 Protein (His tag)

Produktnummer ABIN2745752

Produktdetails

Target: NAPRT1 (NAPRT) (Nicotinate phosphoribosyltransferase (NAPRT))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses NAPRT1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Verwendungszweck: NAPRTase (human) (rec.) (His)

Kreuzreaktivität: Human

Produktmerkmale: Human NAPRTase is fused at the N-terminus to a His-tag.

Reinheit: >98 % (SDS-PAGE)

Biological Activity Comment: 0.043μmol/min/mg: hNAPRTase activity was measured by a custom coupled enzymatic assay which uses a secondary enzyme for the quantification of the PRPP consumed by hNAPRTase. The reaction buffer contained 50 mM Hepes pH 7.5, 500 μM PRPP, 100 μM nicotinic acid, 20 mM MgCl2, 2.5 mM DTT and hNAPRTase at 5 μM, 25°C, spectrophotometrical monitoring.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: In 50 mM HEPES/KOH pH 7.5 containing 300 mM potassium chloride and 10 mM DTT.

Handhabung: After opening, prepare aliquots and store at -80 °C. Avoid freeze/thaw cycles.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung:

Short Term Storage: -20°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.

Haltbarkeit: 6 months

Antigendetails

Target: NAPRT1 (NAPRT) (Nicotinate phosphoribosyltransferase (NAPRT))

Andere Bezeichnung: NAPRTase (NAPRT Produkte)

Hintergrund:

Nicotinate Phosphoribosyltransferase, FHA-HIT-interacting Protein, FHIP, EC 2.4.2.11

Human nicotinate phosphoribosyltransferase (NAPRTase) is localized in the cytoplasm and is involved in biological processes such as NAD biosynthetic and metabolic processes, nicotinamide metabolic process, nicotinate nucleotide salvage, response to oxidative stress and water-soluble vitamin metabolic process. It functions by catalyzing the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN) and is essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells. It is a crucial factor in the NAD+ biosynthesis pathway. Catalytic activity: Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate.

Molekulargewicht: ~58.0kDa

UniProt: Q6XQN6