NAD Synthetase Protein
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- Target
- NAD Synthetase
- Protein-Typ
- Recombinant
- Spezies
- Bacillus subtilis
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Quelle
- Escherichia coli (E. coli)
- Verwendungszweck
- NAD Synthetase (B. subtilis) (rec.)
- Produktmerkmale
- Full-length NAD synthetase (Bacillus subtilis).
- Reinheit
- >98 % (SDS-PAGE)
- Biological Activity Comment
- ~0.3 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1μmol NAD per min.
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Rekonstitution
- 0.5 mg/mL (Lot specific)
- Konzentration
- Lot specific
- Buffer
- In 50 mM TRIS-HCl, pH 8, containing 75 mM sodium chloride, 5 % glycerol and 5 mM DTT.
- Handhabung
- After opening, prepare aliquots and store at -80 °C.Avoid freeze/thaw cycles.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
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Short Term Storage: -20°C
Long Term Storage: -80°C
Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.
- Haltbarkeit
- 6 months
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- Target
- NAD Synthetase
- Hintergrund
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NH(3)-dependent NAD(+) Synthetase, General Stress Protein 38, GSP38, Spore Outgrowth Factor B, EC6.3.1.5
NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It was shown to be induced by heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation and is involved in biological processes like stress response and sporulation resulting in formation of a cellular spore. Catalytic activity: ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+
- Molekulargewicht
- ~30.4kDa
- UniProt
- P08164
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