NADK Protein (His tag)

Produktnummer ABIN2745747

Produktdetails

Target: NADK (NAD Kinase (NADK))

Protein-Typ: Recombinant

Spezies: Mycobacterium tuberculosis

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses NADK Protein ist gelabelt mit His tag.

Verwendungszweck: NAD Kinase (M. tuberculosis) (rec.) (His)

Produktmerkmale: NAD kinase (Mycobacterium tuberculosis) is fused at the N-terminus to a His-tag.

Reinheit: >98 % (SDS-PAGE)

Biological Activity Comment: ~1.2 U/mg protein. (Substrate: NAD (+ ATP)) ~3.8 U/mg protein. (Substrate: NAD (+ poly(P)) One unit is defined as the amount of enzyme that synthesizes 1μmol nicotinamide adenine dinucleotide phosphate (NADP) per min.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: 0.5 mg/mL (Lot specific)

Konzentration: Lot specific

Buffer: In 10 mM TRIS at pH 8.0, containing 1 mM MgCl2, 0.5 mM EDTA, 1 mM DTT and 1 mM PMSF.

Handhabung: After opening, prepare aliquots and store at -80 °C.Avoid freeze/thaw cycles.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung:

Short Term Storage: -20°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.

Haltbarkeit: 6 months

Antigendetails

Target: NADK (NAD Kinase (NADK))

Andere Bezeichnung: NAD Kinase (NADK Produkte)

Hintergrund:

Probable Inorganic Polyphosphate/ATP-NAD kinase, Poly(P)/ATP NAD Kinase 1, ppnK1, EC 2.7.1.23

NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Mycobacterium tuberculosis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Was identified as a high-confidence drug target. Catalytic activity: ATP + NAD+ = ADP + NADP+

Molekulargewicht: ~33.0kDa

UniProt: P0A5S6