EnhancedFasL protein (DYKDDDDK Tag)

Produktnummer ABIN2745658

Produktdetails

Target: EnhancedFasL

Protein-Typ: Recombinant

Spezies: Human, Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: DYKDDDDK Tag

Applikation: Biochemical Assay (BCA), SDS-PAGE (SDS)

Spezifität: Binds to human and mouse Fas (CD95, APO-1).

Kreuzreaktivität: Human, Maus

Bestandteile: 1 Set: 1x10μg of FasL, Soluble (human) (rec.) 4x50μg of TNF Ligands Enhancer

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Source/Host: Produced in HEK 293 cells. A FLAG®-tag is fused at the N-terminus of the extracellular domain of human FasL (aa 103-281). Concentration: FasL: 0.1mg/ml after reconstitution TNF Ligands Enhancer: 1mg/ml after reconstitution Purity: >95% (SDS-PAGE)Formulation: Lyophilized. Contains PBS. Endotoxin Content: FasL: <0.05EU/μg purified protein (LAL test, Lonza). TNF Ligands Enhancer: <0.01EU/μg purified protein (LAL test, Lonza). Reconstitution: FasL: reconstitute with 100μl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions. TNF Ligands Enhancer: reconstitute each vial with 50μl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: Lot specific

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.

Haltbarkeit: 6 months

Antigendetails

Target: EnhancedFasL

Hintergrund: FasL, Soluble (human) (rec.) induces apoptosis in a concentration range of <1 ng/mL in the presence of 0.1 to 1 μg/mL of TNF Ligands Enhancer . (Optimal conditions must be determined individually for each cell line tested)