LTA Protein (Transcript Variant 2)
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- Target Alle LTA Proteine anzeigen
- LTA (Lymphotoxin-alpha (LTA))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- Transcript Variant 2
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Spezies
- Chemical
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Quelle
- Escherichia coli (E. coli)
- Applikation
- Functional Studies (Func), Protein Interaction (PI), Antibody Production (AbP), Standard (STD)
- Spezifität
- Optimal preservation of protein structure, post-translational modifications and functions.
- Produktmerkmale
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- Recombinant human TNF-beta / Lymphotoxin-alpha (transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Reinheit
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin-Niveau
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin-D is less than or equal to 0.05 ng/ml, corresponding to a specific activity of > 2 x 10^7 units/mg.
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- Applikationshinweise
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handhabung
- Resuspend the protein in the desired concentration in proper buffer
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- LTA (Lymphotoxin-alpha (LTA))
- Andere Bezeichnung
- Tnf-Beta,lymphotoxin-alpha (LTA Produkte)
- Substanzklasse
- Chemical
- Hintergrund
- Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. [UniProtKB/Swiss-Prot Function]
- Molekulargewicht
- 18.6 kDa
- NCBI Accession
- NP_000586
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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