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LTA Protein (Transcript Variant 2)

Recombinant LTA-Protein exprimiert in Escherichia coli (E. coli).
Produktnummer ABIN2733938

Kurzübersicht für LTA Protein (Transcript Variant 2) (ABIN2733938)

Target

Alle LTA Proteine anzeigen
LTA (Lymphotoxin-alpha (LTA))

Protein-Typ

Recombinant

Biologische Aktivität

Active

Spezies

  • 10
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Chemical

Quelle

  • 22
  • 6
  • 2
  • 1
  • 1
  • 1
  • 1
Escherichia coli (E. coli)

Applikation

Functional Studies (Func), Protein Interaction (PI), Antibody Production (AbP), Standard (STD)

Reinheit

> 95 % as determined by SDS-PAGE and Coomassie blue staining
  • Proteineigenschaft

    Transcript Variant 2

    Spezifität

    Optimal preservation of protein structure, post-translational modifications and functions.

    Produktmerkmale

    • Recombinant human TNF-beta / Lymphotoxin-alpha (transcript variant 2) protein expressed in E. coli.
    • Produced with end-sequenced ORF clone
    • Tested for bioactivity.

    Endotoxin-Niveau

    Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

    Biological Activity Comment

    ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin-D is less than or equal to 0.05 ng/ml, corresponding to a specific activity of > 2 x 10^7 units/mg.
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  • Applikationshinweise

    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Protein-protein interaction
    In vitro biochemical assays and cell-based functional assays

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Buffer

    Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

    Handhabung

    Resuspend the protein in the desired concentration in proper buffer

    Lagerung

    -80 °C

    Informationen zur Lagerung

    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target

    LTA (Lymphotoxin-alpha (LTA))

    Andere Bezeichnung

    Tnf-Beta,lymphotoxin-alpha

    Substanzklasse

    Chemical

    Hintergrund

    Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. [UniProtKB/Swiss-Prot Function]

    Molekulargewicht

    18.6 kDa

    NCBI Accession

    NP_000586

    Pathways

    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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