Ribonuclease H1 Protein (RNASEH1) (Myc-DYKDDDDK Tag)

Produktnummer ABIN2730997

Dieses Recombinant Ribonuclease H1-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für Ribonuclease H1 Protein (RNASEH1) (Myc-DYKDDDDK Tag) (ABIN2730997)

Target: Ribonuclease H1 (RNASEH1)

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Applikation: Antibody Production (AbP), Standard (STD)

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Produktdetails

Aufreinigungstag / Konjugat: Dieses Ribonuclease H1 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Produktmerkmale:

• Recombinant human RNASEH1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: Ribonuclease H1 (RNASEH1)

Andere Bezeichnung: Rnaseh1

Hintergrund: This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and is necessary for DNA replication and repair. This enzyme is present in both mitochondria and nuclei, which are resulted from translation of a single mRNA with two in-frame initiation start codons. The use of the first start codon produces the mitochondrial isoform and the use of the second start codon produces the nuclear isoform. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF) which overlaps the first initiation start codon in human. An alternately spliced transcript variant has been found which encodes a shorter isoform. This gene has three pseudogenes two of them are at different locations of chromosome 17 and one of them is on chromosome 1q32.2.

Molekulargewicht: 31.9 kDa

NCBI Accession: NP_002927