Nucleolin Protein (NCL) (Myc-DYKDDDDK Tag)

Produktnummer ABIN2727708

Produktdetails

Target: Nucleolin (NCL)

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Nucleolin Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human Nucleolin protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Referenzen

Hernández, Sandoval-Jaime, Sosnovtsev, Green, Gutiérrez-Escolano: "Nucleolin promotes in vitro translation of feline calicivirus genomic RNA." in: Virology, Vol. 489, pp. 51-62, (2016) (PubMed).

Perrone, Butovskaya, Lago, Garzino-Demo, Pannecouque, Palù, Richter: "The G-quadruplex-forming aptamer AS1411 potently inhibits HIV-1 attachment to the host cell." in: International journal of antimicrobial agents, Vol. 47, Issue 4, pp. 311-6, (2016) (PubMed).

Tosoni, Frasson, Scalabrin, Perrone, Butovskaya, Nadai, Palù, Fabris, Richter: "Nucleolin stabilizes G-quadruplex structures folded by the LTR promoter and silences HIV-1 viral transcription." in: Nucleic acids research, Vol. 43, Issue 18, pp. 8884-97, (2015) (PubMed).

Antigendetails

Target: Nucleolin (NCL)

Andere Bezeichnung: Nucleolin (NCL Produkte)

Synonyme: C23 Protein, B530004O11Rik Protein, D0Nds28 Protein, D1Nds28 Protein, Nucl Protein, wu:fa12d03 Protein, zgc:152810 Protein, nucl Protein, LOC100282923 Protein, LOC100283058 Protein, nucleolin Protein, nucleolin S homeolog Protein, NCL Protein, Ncl Protein, ncl.S Protein, ncl Protein, LOC100195537 Protein, LOC100282923 Protein, LOC100283058 Protein

Hintergrund: Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

Molekulargewicht: 76.4 kDa

NCBI Accession: NP_005372

Pathways: Ribonucleoprotein Complex Subunit Organization