NMT1 Protein (Myc-DYKDDDDK Tag)

Produktnummer ABIN2727416

Recombinant NMT1-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für NMT1 Protein (Myc-DYKDDDDK Tag) (ABIN2727416)

Target: NMT1 (N-Myristoyltransferase 1 (NMT1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Applikation: Antibody Production (AbP), Standard (STD)

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Produktdetails

Aufreinigungstag / Konjugat: Dieses NMT1 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Produktmerkmale:

• Recombinant human NMT1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: NMT1 (N-Myristoyltransferase 1 (NMT1))

Andere Bezeichnung: Nmt1

Hintergrund: Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1 MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM, Nov 2008].

Molekulargewicht: 56.6 kDa

NCBI Accession: NP_066565

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling