MMP1 Protein (Transcript Variant 1)

Produktnummer ABIN2726136

Produktdetails

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: Transcript Variant 1

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human MMP-1 (transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Reinheit: > 90 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin-Niveau: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: MMP-1 activity was measured by its ability to cleave a chromogenic peptide MMP-1 substrate at room temperature. At an MMP-1 concentration of 2.5 ug/mL, 50% cleavage was achieved at an incubation time of approximately 25 minutes

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handhabung: Resuspend the protein in the desired concentration in proper buffer

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Andere Bezeichnung: Mmp-1 (MMP1 Produkte)

Hintergrund: This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Molekulargewicht: 42.7 kDa

NCBI Accession: NP_002412